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It creates a high risk of contamination that can lead to skewed data or failed experiments
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Furthermore, local targeting of NAD + salvage pathway by microparticle-mediated intratumoral delivery of NAMPT inhibitor GMX1778 induced striking immunologic changes in the tumor microenvironment of murine glioblastoma, characterized by upregulation of immune checkpoint PD-L1, recruitment of cytotoxic T cells, and reduction of tumor-associated macrophages, and potentiated checkpoint immunotherapy [114]
