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This approach consists of three main steps, alkylating reduced Cys in proteins with a probe, click chemistry-based incorporation of isotopically-labeled cleavable tags, protein purification and on-bead digestion (Weerapana et al., 2007)

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[DOI] [PubMed] [Google Scholar] 25.Bialonska D., Ramnani P., Kasimsetty S.G., Muntha K.R., Gibson G.R., Ferreira D

Chronic airway inflammation, oxidative stress, and systemic spillover of inflammatory mediators like IL-1, IL-6, and TNF- are all carried by chronic exposure to inhaled irritants, particularly cigarette smoke and pollution