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A data review identified serious adverse events associated with chelation therapy in ASD, such as hypocalcemia, renal impairment, and reported deaths, concluding that the risks currently outweigh any proven benefits [129]

and third, whether the stimulation of mitochondrial biogenesis by PGC-1 is a possible underlying mechanism for their preventative effects

Lipofectamine 2000 was purchased from Invitrogen (Carlsbad, CA, USA) and OPTI-MEM Reduced Serum Medium were purchased from Gibco (Langley, USA)

There is also some evidence that ALA can reduce the advanced glycation end products in neuronal cells with promising results for the treatment of Alzheimers disease [27]