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The supernatant was transferred into a 96-well plate and incubated with the assay cocktail containing MES-buffer, co-factor and enzyme mixture, and Ellman's reagent for 30 min

For example, GAPDH that plays a role in mediating ROS signaling in plants is a direct target of H 2 O 2 and it is also a target of NO-mediated S -nitrosylation, which blunts its activity (Lindermayr et al., 2005)

CNF was analyzed by using high performance liquid chromatography (HPLC) and ultraviolet absorption spectroscopy for its drug content determination

A custom workflow reveals that nucleolar protein pyrophosphorylation in human cells regulates the transcription of ribosomal DNA