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pH 7.0) to which the following solutions were added: 25 L of glutathione reductase solution (100 /mL), 25 L of sodium azide (0.12 m), 50 L Na 2 EDTA (0.15 mm), 50 L of NADPH (3 mm), and 50 L aliquots of supernatant obtained after centrifugation at 14,000 g for 25 min

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2.2.4 The role of hypoxic condition in the TME in regulating the epigenetic reprogramming of Tregs Hypoxia is a prevalent characteristic of numerous solid tumors (Macedo-Silva et al., 2019)