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The mutations generated by Cas9 at the target exons were obtained by preparing genomic DNA from the selected clones and amplifying the exons with the primers: Fwd (hA5-ck1): 5-TCTTGTATCTGTACCTCTGA-3

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Probably in the past, like two or three years, I feel like the years of training, um, have sort of caught up to me a little bit
