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Description
103 (8), e31
The following primers were used: mFIAF-PPRE (forward primer 5-GGCAGACCCAGAAAGATGG-3, reverse primer 5-CCGATTGGATGAGAGGAAAG-3) mADRP-PPRE (forward primer 5-GCTGGGGATTACAGACCAGA-3, reverse primer 5-TCTTGGGGTTTTGGAAAATG-3) Transactivation assays COS-7 cells were cultured in 12-well plates and co-transfected with PPRE X3-TK-luc, pRL-TK, pXJ40-3xFlag-FABP5 WT, or C127S, according to the Lipofectamine reagent protocol (Invitrogen, 11668-019)
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