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Finally, the fermentation was scaled up in a 5-L fermentor, and the highest titer and yield were improved to 5680 mg/L and 45.13 mg/g, respectively, by optimizing the addition conditions of amino acids (40 mM added after 40 h)

In situ addition of [Pd(allyl)Cl] 2 and GSH in 6 M GnHCl to the reaction mixture enabled subsequent efficient Thz opening within 45 min (Fig

10.1073/pnas.1216100110 87
