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Aguirrezabalaga I
This study was supported in part by the Spanish Ministry of Health through an official grant from the Fondo de Investigacin Sanitaria (FIS00/0435), Madrid, Spain

It is intended only for properly supervised in vitro experimentation or authorized non-human preclinical research conducted by trained and qualified personnel

Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution