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After centrifugation (1200 g , 4 C, 10 min) 9 mL acidified H 2 O was added (final pH = 3.5) and samples were subjected to solid phase extraction

To calculate the relative concentration of cytosolic ROS, all images were first converted to 32-bit format and thresholded with values below the threshold set to Not a Number

Glycolysis is crucial for NK cell cytotoxicity, and in the AML microenvironment, glucose restriction dramatically lowers the synthesis of perforin/granzymes, which eventually results in the loss of NK cells ability to kill (111)

It is used when the cause or specific type of elevated white blood cells is unknown