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The gene, including its stop codon, was cloned into the pPD95_77 plasmid using the restriction sites SalI and KpnI

In a UK survey, the vast majority (92%) of owners of cats treated were happy with the choice of radioiodine therapy, and the major barrier to treatment was concern about hospitalisation time, and concern about missing their pet or their cat being unhappy 6

Of note is that although the C/EBP promoter contains no STAT3 DNA binding motif sequences, a novel mechanism indirectly tethering STAT3 to the C/EBP promoter has been described.2426 This result underscores the important role of STAT3 in orchestrating the transforming capacity of NPM-ALK and support studies suggesting STAT3 as a possible therapeutic target.27 C/EBP activity is further regulated through specific phosphorylations that determine its transcriptional activation and DNA binding capacity.24,28,29 The ERK1/2-dependent phosphorylation of human LAP at Thr 235 is a critical priming site required for the transcriptional activation of C/EBP.30 In contrast to a previous study,8 we did not find down-regulation of total C/EBP protein upon pharmacological inhibition of ERK1/2 activation

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