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Dip each teat so the lower 1 inch of the teat is coated with the 0.5% solution

After the initial appearance of ovarian tissue, ten consecutive sections were collected, and the 1st, 5th, and 10th of these were selected for hematoxylin-eosin staining and histological evaluation

The generated peptides were eluted from the gel pieces with 1% (v/v) formic acid (FA) in 60% (v/v) acetonitrile, dried down by vacuum centrifugation, and finally dissolved in 1% (v/v) FA