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Mol Plant Microbe Interact 18(3):254259

Cells were washed three times with ice-cold PBS and lysed in lysis buffer (40 mM Tris-HCl (pH 8.0), 1% Triton X-100, 1% NP-40, 10% glycerol, 0.15 M NaCl, 2 mM EDTA, 1 mM phenylmethanesulfonyl fluoride, 1 Complete protease inhibitor cocktail)

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Finally, they were combined, concentrated and freeze-dried to obtain 13.6 g of the finished product, and the purity determined by HPLC was greater than 98%, indicating that this technical condition can be directly scaled up for production