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Description
Briefly, in the presence of 150 g/mL fibrinogen-FITC and 2.5 mmol/L CaCl 2 , PRP or gel-filtered platelets diluted in buffer to a concentration of 2.5 10 7 platelets per mL were incubated with PVL or bacterial supernatant for 60 min at room temperature with or without addition of isolated neutrophils, fixed with 1% paraformaldehyde and analysed with fluorescence-activated cell scanner (FACSCalibur, Becton Dickinson)
Endocrine 27 (2), 119130
doi: 10.1016/0006-2952(88)90591-6
Multiply your ml volume by 100
