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The reaction between RT and GSH is based on a Michael addition reaction that is inherently reversible, has an appropriate dissociation equilibrium constant K d in the mM range and rapid reaction kinetics, and provides ratiometric readouts, thus allowing GSH quantification independent of the probe concentration
Phosphorylation of IB by the IKK complex triggers its recognition by an E3 ligase complex which leads to its polyubiquitination and subsequent degradation by the proteasome
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