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doi: 10.3390/molecules29143328 131

In brief, blood was drawn by median cubital vein venipuncture from healthy volunteers and centrifuged through a Ficoll Histopaque 1077 gradient (Sigma) to isolate mononuclear cells

Deficiencies occur when supplies are reduced to 300 g of B 12 per day

KD ameliorated UC-induced intestinal barrier damage by inhibiting the overactivation of the PI3K/Akt/NF-B signaling pathway, attenuating the inflammatory response, including restoration of the intestinal villi and mitochondrial structure, and modulating the gut microbiota (increasing Alloprevotella , Treponema , Prevotella and Prevotellaceae , and decreasing Escherichia_Shigella and Desulfovibrio ) to optimize further the intestinal microenvironment (Liu et al., 2020)