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10.1002/cbdv.202300615 165 ZangM.ZhuF.ZhaoL.YangA.LiX.LiuH.et al (2014)

It did not include histopathological analysis of brain tissue, which could confirm structural changes related to neurochemical and oxidative shifts

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To achieve this, we employed an iterative registration framework called Elastix (43), complemented by a Python wrapper package named Pyelastix (accessible at The transformation parameters were computed based on the nuclear channel obtained in each cycle, where the initial cycle served as the reference to which all subsequent cycles were aligned