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Then, HUVECs were trypsinized and resuspended in the conditioned medium collected from different treated THP-1 cells at 2 10 5 cells/ml
However, samples with protein concentrations exceeding 3 g/dl must be diluted using a 0.1 M phosphate buffer at pH 7.0 to obtain reliable results
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2021 Dec;118(12):4687-4698
