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To determine whether Ssu72 functions as a phosphatase for GSK3, purified GST-GSK3 was phosphorylated by recombinant AKT and then incubated with purified His-Ssu72 (WT) or His-Ssu72 phosphatase-dead mutant (C12S) proteins (Supplementary Fig
Zinc is found in oysters, meat and seafood, as well as seeds and nuts, although zinc is less able to be absorbed from non-animal sources
