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Kerstjens HA, Rijcken B, Schouten JP, Postma DS
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For transient transfection, plasmids were transfected into HEK293T cells using polyethylenimine (PEI, branched, Sigma-Aldrich, Germany)
Studies show that increasing GABA activity in the brain may restore the neurotransmitter system to equilibrium, which may lessen insomnia symptoms
