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For the western blotting of HMGB1, frozen cortices were homogenized in 50 mM TrisHCl (pH 7.5) containing protease inhibitor cocktail and phosphatase inhibitor cocktail

Several, independent meta-analyses have reported that the majority of patients with bipolar disorder experience problems with memory loss and cognition across all phases of the disorder, even during periods of remission in a manner that increases with recurrence [101,102,103]

Similarly, copper accumulation could lead to abnormal oxidative metabolism, attacking to the structures and functions of biomolecules such as proteins, which could lead to the occurrence of diseases such as diabetes, neurological disorders, and cardiovascular dysfunctions (Shi et al., 2023

In some individuals with persistent, debilitating fatigue following SRAS-CoV-2 infection, documented damage of vital organs may be a sufficient explanation for their fatigue
