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General mitochondrial function was assessed based on the Seahorse XF Cell Mito Stress Test protocol (Agilent Technologies, Santa Clara, CA, USA)

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Previous studies showed that it did not significantly suppress the growth of KRAS-derived PDAC cells in vitro or in vivo because these cells mounted an adaptive metabolomic response, suggesting the importance of combined therapy for avoiding metabolic adaptation in response to GLS inhibition 45

Among the most widely used agents to maintain the cysteine pool is NAC in addition to -lipoic acid, which will not be discussed in detail