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Rats were purchased from the Comparative and Experimental Medical Center of the Shiraz University of Medical Sciences (SUMS)
For bacterial diversity analysis, V3-V4 variable regions of 16S rRNA genes were amplified with universal primers 343F (5-TACGGRAGGCAGCAG-3) and 798R (5-AGGGTATCTAATCCT-3) ( 2.7 SCFA concentration analysis The levels of SCFAs in the sera of mice were measured with GCMS analysis

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