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Each 2mg injection contributes approximately 0.03mg copper, compared to 0.9-2.2mg from typical daily diet

Bone marrow cells were cultured for 6 d in a complete Roswell Park Memorial Institute (RPMI) 1640 medium containing 10 % fetal bovine serum (FBS), 10 ng/mL recombinant mouse granulocytemacrophage colony-stimulating factor (GM-CSF) (RP-8620, Invitrogen) and 10 ng/mL recombinant mouse IL 4 (404-ML/CF, RD) in 37 C, 5 % CO 2 incubator

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A decrease in NAD+ adversely impacts cellular energy metabolism, while a reduction in SAM hinders the activation of genes that are vital for boosting metabolism