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To purify secreted proteins that are produced from eukaryotic cell cultures (such as insect cells, CHO cells, 293 cells etc.), a step of removing incompatible agents to Ni-NTA resins by dialysis is frequently required prior to loading the material on the column
Esterbauer H, Schaur RJ, Zollner Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes
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