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While performing metabolome analysis of 10 brain regions of C57BL/6N mice over 4 time points across the lifespan, another group studying aging-related metabolite level changes in the brain found very different results in how the pyruvate levels changed with aging (Ding et al., 2021)

As a result, the expression of epigenetic downstream targets, notably Pdgfraa gene essential for facial developmentwas impeded, leading to severe defects in midline facial development in the majority of the mice [180]

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