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Nickel beads are blue (left), but that color can change during protein purification. If the blue color doesnt return and your column is still white after elution, this means that you stripped the nickel ions off of the agarose beads, likely by using too much EDTA in your buffers

Means and variances (SDS) were extracted for each measure to calculate effect sizes

The hypoxia alleviation via O2 production, the redox balance disruption via glutathione consumption and the reactive oxygen species generation, together would down-regulate the expression of O6-methylguanine-DNA methyltransferase under TMZ medication, which is considered as the key to drug resistance

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