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Margraf A, Ley K, Zarbock A (2019) Neutrophil recruitment: from model systems to tissue-specific patterns

10.1016/j.jneuroim.2009.12.003

For in-gel phosphoprotein staining, equal quantities (5 g) of untreated Ycf1 and lambda PP (NEB) treated samples were separated on two separate 10% SDS- PAGE gels, one of which was then stained with Pro-Q Diamond Phosphoprotein Gel Stain (Thermo Scientific) following the manufactures protocol to detect protein phosphorylation
