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Unbroken cells and cell debris were removed by centrifugation at 13,000 g for 10 min at 4 C
Numerous studies have aimed at assessing the effects of IGF1 on mitochondrial biogenesis

It acts faster on fat mass than MOTS-c but does not address insulin sensitivity or mitochondrial function

After 812 h, the microbial metabolites 5-(3,4-dihydroxyphenyl)--valerolactone and 5-(3-methoxy-4-hydroxyphenyl)--valerolactone (see section 6.1 Pre-systemic metabolism: role of gut microbiota) were present in urine (30)
