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Description
A speculum widens your vaginal walls just enough for your provider to see your vaginal canal and your cervix
Afterwards, tubes were briefly centrifuged and real-time PCR was carried out in a 7500 Real-Time PCR System (Applied Biosystems, Foster, CA, USA) using the following cycling parameters: 2 min at 50C

M., Heinlen, L

The fluorescence anisotropy (A) was calculated with MARS Data Analysis software (BMG Labtech) using the equation (I parallel I perpendicular )/(I parallel +2xI perpendicular )
