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AOD-9604 works by activating beta-3 adrenergic receptors that are highly expressed in adipose (fat) tissue but have minimal presence in appetite-regulating brain regions

After the incubation, cell viability was evaluated using the CCK-8 assay and absorbance was measured at 450 nm using a Victor X4 microplate reader (PerkinElmer, USA)

However, a recent systematic review of 20 randomized controlled trials (RCTs) ( n = 2256) 62 did not substantiate the potential of riboflavin as adjuvant for depressive symptom alleviation

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