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Based on the above mechanisms, the synergy between ferroptosis and immune checkpoint inhibitors constitutes a powerful positive feedback loop

Most compounds that target tumor metabolism (methotrexate, 5-fluorouracil) fail to distinguish between tumor tissue and rapidly differentiating epithelial tissue (skin, bone marrow), 4,322 whereas therapies targeting the specific amino acid dependence of tumor cells are cell-selective, such as leukemic blasts that are selectively dependent on Asn, the use of bacterial-derived ASNase in pediatric ALL has significantly improved the cure rate

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Samples were incubated in a Thermoshaker for 30 min at 1.200 rpm / 4C, vortexed vigorously and spun down for 10 min at 12,000g / 4C