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Mounce BC, Cesaro T, Carrau L, Vallet T, Vignuzzi M
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The hydrolysis process of p-nitrophenylphosphate in alkaline buffer solution at pH of 10.3 and a temperature of 37 C was determined, through colorimetric evaluation of the biproduct (p-nitrophenol) in an ELISA plate reader at 400 nm (Synergy HT, Biotek)

Allergic Reactions: In rare cases, individuals may have allergic reactions to B12 injections, ranging from mild skin irritation to severe anaphylaxis
