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Elevated NNMT is linked to: Reduced NAD+ availability Slower mitochondrial function Increased fat storage efficiency Higher risk for insulin resistance By reducing NNMT activity, 5-Amino-1MQ allows the body to maintain higher NAD+ levels, which can support better mitochondrial performance, improved fat oxidation, and more balanced energy metabolism

These findings suggest that both NNMT and its product MNA increase Sirt1 protein expression, while Sirt1 is needed for the regulatory effects of NNMT and MNA on glucose metabolism [14]

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Ruf S, Rajagopal S, Kadnur SV, Hallur MS, Rani S, Kristam R, Swaminathan S, Zope BR, Gondrala PK, Swamy I (2022) Novel tricyclic small molecule inhibitors of Nicotinamide N -methyltransferase for the treatment of metabolic disorders